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KMID : 0366119860140060479
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Korean Journal of Applied Microbiology & Bioengineering
1986 Volume.14 No. 6 p.479 ~ p.486
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Cloning and Expression of A Liquefyiog α - Amylase Gene from Bacillus amyloliquefaciens in Bacillus subtilis
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金思悅/Ghim, Sa-Youl
宋邦鎬/李燐九/徐正塤/洪淳德/Song, Bang-Ho/Rhee, In-Koo/Seu, Jung-Hwn/Hong, Soon-Duck
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Abstract
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A 5200 basepair DNA fragment containing the Bacillus amvloliquelociens amyE gene, encoding liquefying a-amylase (1,4-a-1)-glucan glucanohydrolase, EC 3.2.1.1), has been inserted into Ban1Hl site of the pUB110 and the hybrid plasmid was designated as pSKS3. The pSKS3 was transformed into the Bacillus sublilis KM213 as a host which is a saccharifying a-amylase deficient mutant of Bacillus sublilis NA64, and the plasmid in the transformed cell was ex-pressed a-amylase production and kanamycin resistance. The a-amylase production of the transformed cell was reduced to one fifth of that of the donor strain. The Bacillus sublilis KM213 earring pSKS3 indicated that the amvE gene pruduct is a polypeptide which has the same electrophoretic mobility with that of the Bacillus omyloliqueluciems, but different from the saccharifying a-amylase of Bacillus sublilis NA64. It means that the umyE gene of pSKS3 originales from the Bacillus amylo[iquefaciens.
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